Cloned (Comment) | Organism |
---|---|
expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
11-amino-undecanoyl-RARRRKKRT | - |
Homo sapiens | |
4,6-bis(4-guanidinylphenoxy)-1-guanidinyl-3-(4-guanidinylphenylamino)cyclohexane | - |
Homo sapiens | |
8-amino-octanoyl-RARRRKKRT | - |
Homo sapiens | |
acetyl-RARRRKKRT | - |
Homo sapiens | |
beta-Ala-TPRARRRKKRT-amide | - |
Homo sapiens | |
cholyl-RARRRKKRT | - |
Homo sapiens | |
decanoyl-Arg-Val-Lys-Arg-chloromethylketone | - |
Homo sapiens | |
additional information | EC values of furin inhibitors, overview. Inhibition of cell-surface PA83 processing in cell-based assays, overview | Homo sapiens | |
N-[5-guanidino-2,4-bis-(4-guanidino-phenoxy)-cyclohexyl]-guanidine | - |
Homo sapiens | |
N-[5-guanidino-2,4-bis-(5-guanidino-pyridin-2-yloxy)-cyclohexyl]-guanidine | - |
Homo sapiens | |
RARRRKKRT | - |
Homo sapiens | |
TPRARRRKKRT | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Homo sapiens | 9986 | - |
Golgi apparatus | - |
Homo sapiens | 5794 | - |
additional information | furin is present both in the intracellular secretory pathway and at the cell surface | Homo sapiens | - |
- |
secretory vesicle | - |
Homo sapiens | 99503 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
membrane type-1 matrix metalloproteinase proenzyme + H2O | Homo sapiens | intracellular processing in breast carcinoma MCF-MT1-E240A-FLAG cells | membrane type-1 matrix metalloproteinase + propeptide of membrane type-1 matrix metalloproteinase | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant furin from the stably transfected Sf9 insect cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
glioma cell | - |
Homo sapiens | - |
HT-1080 cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
U-251 MG cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthrax protective antigen-83 + H2O | - |
Homo sapiens | ? | - |
? | |
glycoprotein 160 + H2O | from HIV-1, low activity | Homo sapiens | ? | - |
? | |
hemagglutinin + H2O | from avian influenza H5N1 virus, high activity | Homo sapiens | ? | - |
? | |
membrane type-1 matrix metalloproteinase proenzyme + H2O | - |
Homo sapiens | membrane type-1 matrix metalloproteinase + propeptide of membrane type-1 matrix metalloproteinase | - |
? | |
membrane type-1 matrix metalloproteinase proenzyme + H2O | intracellular processing in breast carcinoma MCF-MT1-E240A-FLAG cells | Homo sapiens | membrane type-1 matrix metalloproteinase + propeptide of membrane type-1 matrix metalloproteinase | - |
? | |
pyroglutamic acid-RTKR-4-methylcoumarin 7-amide + H2O | - |
Homo sapiens | pyroglutamic acid-RTKR + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Proprotein convertase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000065 | - |
acetyl-RARRRKKRT | pH 7.5, 37°C | Homo sapiens | |
0.000008 | - |
RARRRKKRT | pH 7.5, 37°C | Homo sapiens | |
0.0000083 | - |
8-amino-octanoyl-RARRRKKRT | pH 7.5, 37°C | Homo sapiens | |
0.0000089 | - |
11-amino-undecanoyl-RARRRKKRT | pH 7.5, 37°C | Homo sapiens | |
0.0000152 | - |
cholyl-RARRRKKRT | pH 7.5, 37°C | Homo sapiens | |
0.000023 | - |
TPRARRRKKRT | pH 7.5, 37°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000023 | - |
pH 7.5, 37°C | Homo sapiens | beta-Ala-TPRARRRKKRT-amide |
General Information | Comment | Organism |
---|---|---|
physiological function | furin and related proprotein convertases cleave the multibasic motifs R-X-R/K/X-R in the precursor proteins and, as a result, transform the latent proproteins into biologically active proteins and peptides | Homo sapiens |